By Tom Fairless 

BRUSSELS--The European Union escalated its five-year antitrust probe into Google Inc. Wednesday, accusing the U.S. search giant of violating the bloc's antitrust laws and opening a formal probe aimed at Google's Android operating system for mobile phones.

The charges from the EU's competition regulator are a blow for Google, reopening the prospect of large fines and injunctions that could severely restrict how the U.S. firm does business in Europe.

It is the first time any regulator has filed formal antitrust charges against Google, putting the EU in the vanguard of a global debate over the regulation of giant Internet platforms.

The charges "just put [Google] in a worse position, by reducing their leverage in bargaining" with EU regulators, said Keith N. Hylton, a law professor at Boston University.

However, the charges based around Google's comparison shopping services were more limited than complainants had demanded.

Both sides appeared to sharpen their rhetoric on Wednesday, indicating that the case could be headed for the courts rather than a fresh settlement proposal--though that remains a possibility.

At a crowded news conference, Margrethe Vestager, the EU's new antitrust chief, said she had reached the preliminary conclusion that Google abuses its dominant position in online search to systematically favor its own comparison shopping service, Google Shopping, over rival services on its general search page. That conduct started in 2008, the EU said.

"I'm concerned that Google has artificially boosted its presence in the comparison shopping market with the result that consumers do not see what's relevant for them," Ms. Vestager said.

In a statement posted online, Google said it strongly disagrees "with the need to issue a statement of objections and look forward to making our case over the weeks ahead." A statement of objections is the formal name for EU antitrust charges.

"While Google may be the most used search engine, people can now find and access information in numerous different ways--and allegations of harm, for consumers and competitors have proved to be wide of the mark. In fact, people have more choice than ever before," Google said in an online blog.

It said there was "a ton of competition" in online shopping sites such as Amazon and eBay. And while complainants like Expedia and TripAdvisor have claimed Google's practices have hurt their results, it said their traffic, revenues and profits "tell a very different story."

By focusing the charges exclusively on comparison shopping, the EU significantly narrowed the scope from its previously expressed concerns around Google's business practices.

If the EU proves that Google has violated competition law in comparison shopping, that "could potentially establish a broader precedent" that would also impact other fields, such as travel websites, Ms. Vestager said.

She said her agency would continue to look at Google's conduct in other areas, such as its treatment of specialist search services for "hotels or flights or maps", allegations that Google copies or "scrapes" content from rival sites, its exclusivity contracts with advertisers, and advertisers' ability to use competing platforms.

"I will carefully consider [Google's] response before deciding how to proceed," she said.

Asked whether a settlement with Google was likely, Ms. Vestager said that "every road is open at this point, both when it comes to commitments, but also when it comes to the other road...which is a fine."

But she indicated that any fresh proposal would need to differ substantially from three earlier settlement attempts, all of which failed and caused lengthy delays.

Early last year, Google came tantalizingly close to a deal with regulators, only to watch it unravel over the summer in the face of huge political pressure and opposition from the companies it was designed to protect.

Google's previous attempts to settle the EU's concerns were based on tweaks to its search page, such as reserving space near the top of the page for competitors to serve specialized search results for things like hotel rooms alongside Google services that did the same thing.

That type of solution, Ms. Vestager said, was unlikely to cut it this time.

"I'm looking for something based on principle and hopefully future-proof, " she said. "For me, the design of the screen that meets you is also an innovative process. For me, it would be better to have a solution based on principle rather than a particular look of the screen."

Formal charges against Google seemed far-fetched as recently as last summer, after the EU's former antitrust chief JoaquĆ­n Almunia publicly backed a settlement with the U.S. company early last year over strong objections from complainants.

Mr. Almunia subsequently announced an about-face in September, following intense political pressure from powerful actors including German publishing houses and the economy ministers of France and Germany.

"They were so close [to a deal] and suddenly it fell apart," Mr. Hylton said.

News Corp, publisher of The Wall Street Journal, on Tuesday joined a group of companies that have filed formal complaints with the commission regarding Google's competition practices, a spokesman said. He declined to disclose further details.

Experts said it would be difficult to strike a settlement that satisfies complainants and doesn't present a real hindrance to Google.

"Google are revising their algorithms all the time and made clear that any kind of regulatory solution would be complicated and get in the way of their business," Mr. Hylton said.

A final EU decision could follow later this year after Google is given time--probably about three months--to make its case to regulators, and could influence regulators in other jurisdictions, experts said.

The decision to investigate Google's conduct in relation to Android is important as an ever-greater share of Internet usage shifts to mobile devices, Ms. Vestager said.

The investigation will focus on whether Google leveraged its market power to illegally hinder rival mobile operating systems and mobile applications, for instance by requiring manufacturers to pre-install its own applications and by bundling its products with other Google services.

EU regulators have received a number of complaints relating to Google's business practices around Android, and had been carrying out an informal investigation.

"My wish is that consumers benefit from the broadest possible range of mobile services," Ms. Vestager said.

Google said its partner agreements with Android were voluntary--you can use Android without Google--but provide real benefits to Android users, developers and the broader ecosystem. It also said its app distribution agreements with manufacturer made sure "people get a great 'out-of-the-box' experience with useful apps right there on the home screen."

Write to Tom Fairless at tom.fairless@wsj.com

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