WASHINGTON--The Federal Reserve took enforcement action against Banco Santander SA's U.S. unit, saying one of the Spanish bank's subsidiaries paid a dividend without permission even though the Fed had barred it from doing so.

Santander Holdings USA, Inc. has been under dividend restrictions since it failed a U.S. "stress test" in March. The rejection stemmed from qualitative problems the Fed found with the bank's stress-test program, such as measurements of potential risks and losses.

A Santander spokesman declined to comment.

It is the first time the Fed has taken stress-test related enforcement action against a bank.

The Fed released a written agreement with the U.S. unit of Santander Thursday that bars the bank from declaring or paying dividends without the regulators' prior approval. The Fed gave Santander 30 days to submit a plan for ensuring its board of directors has oversight of dividend payments.

Banco Santander, the parent company of Santander USA, has contributed $21 million to the U.S. unit to mitigate the violation, the agreement said.

The Fed's action is the latest tussle between the regulator and banks based outside the U.S. The Fed is trying exert more authority over those banks' U.S. units and recently subjected them to rules requiring they maintain higher minimum capital levels. It also puts those banks through the annual stress test exercise--which looks at a banks' ability to weather a recession--for the first time earlier this year.

Write to Ryan Tracy at ryan.tracy@wsj.com

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