Health workers trying to tackle the Ebola virus outbreak that has killed more than 1,400 people in West Africa are faced with diminishing options to gain access to the region after Air France on Wednesday said it would suspend flights to Freetown, Sierra Leone.

Air France said it would halt flights from Aug. 28 on instructions from the French government. France also told nonessential personnel to leave Sierra Leone and Liberia. With the endorsement of the French government, Air France will continue to serve the capital cities of Guinea and Nigeria, which also have been hit by Ebola.

Medical personnel are finding it increasingly difficult to manage staffing. British Airways suspended service to Liberia and Sierra Leone early this month over Ebola concerns and now has extended the operational hiatus until year's end "due to concerns about the health facilities in both countries." Emirates Airline, the largest international carrier by traffic, stopped serving Conakry, Guinea weeks ago.

The reduction in international air service is hurting efforts to get medical teams and supplies to Liberia, Sierra Leone and Guinea, said Brice de le Vingne, director of operations for Médecins sans Frontières, a not-for-profit group providing medical assistance in conflict and disaster areas. MSF is the largest provider of international medical staff in the current Ebola outbreak.

"Look at the de facto quarantine this is putting on these countries," he said. "This is terrible." MSF staff are rotated every four weeks, so "we need air transport," he said.

A World Health Organization worker infected with Ebola on Wednesday was flown to Hamburg on a specially equipped private jet for treatment. The U.K. over the weekend used a specially configured Boeing C-17 military airlifter from the Royal Air Force to repatriate a health-care worker who fell sick from Ebola in Sierra Leone.

The ability to charter private planes, a more expensive way to travel, also has been impacted. Many operators have pulled their planes out of West Africa making it more difficult to arrange short term rentals, an industry official said.

The Ebola outbreak has killed more than 1,420 people and infected more than 2,600 as of Aug. 20. There is no vaccine for the viral hemorrhagic fever that can puncture blood vessels to cause internal bleeding. The World Health Organization has recorded the most Ebola-linked deaths in Liberia, followed by Guinea, Sierra Leone, and Nigeria. Two Ebola cases were recorded this week in the Democratic Republic of Congo in an unrelated outbreak.

Brussels Airlines and Royal Air Maroc are among the airlines still serving the region. A Brussels Airlines spokeswoman said the carrier would continue serving Conakry, Freetown and the Liberian capital, Monrovia, while monitoring the situation. Brussels Airlines has some of most-extensive experience flying into Ebola-affected regions and has been working with the Institute of Tropical Medicine in Antwerp to assess the situation.

Delta Air Lines in July said it would stop flying to Monrovia at the end of August citing weak demand, rather than the viral outbreak. Its services to Lagos, Nigeria; Dakar, Senegal; and Accra, Ghana, continue. United Continental Holdings also is continuing its flights to Lagos.

Intra-African flights also have been restricted in the wake of the outbreak. Ivory Coast has blocked flights from Ebola-stricken countries and Nigeria's civil-aviation authority also has restricted some flights.

The flight suspensions come even after the International Civil Aviation Organization, the United Nations' aviation-safety arm, said this month that the risk to passengers of becoming infected on flights was low. Transmission requires direct contact and those infected with Ebola are generally too ill to travel.

Air France said it suspended service on instructions from the French government, rather than out of concern about procedures in place in Sierra Leone. Regional authorities have begun screening passengers to ensure those infected don't board flights.

Susan Carey and Drew Hinshaw contributed to this article.

Write to Robert Wall at robert.wall@wsj.com and Drew Hinshaw at drew.hinshaw@wsj.com

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