SEOUL-- Samsung Electronics Co. said Wednesday that it had acquired a U.S. startup whose technology is likely to underpin the South Korean technology giant's new mobile-payment service.

The deal to buy LoopPay Inc. of Burlington, Mass., represents the clearest signal that Samsung intends to compete aggressively with Apple Inc. in trying to change consumer behavior at the checkout counter.

For Samsung, the LoopPay acquisition marks a further foray into building up an ecosystem of software and services that can set its smartphones apart from an increasingly crowded field of handset makers running Google's Android platform.

"We've been very focused on the next generation of software and services, " said David Eun, the Samsung executive who spearheaded the deal. In an interview, Mr. Eun said LoopPay would join Samsung's mobile division, which is building a platform to provide consumer services related to payments, including tracking loyalty points and spending habits.

The acquisition of LoopPay for an undisclosed amount will pit the South Korean smartphone maker against other technology giants with mobile payment services, including Google Inc., eBay Inc.'s PayPal and Apple Pay, which started in the U.S. last fall.

Like many of the other offerings on the market, LoopPay's technology would allow a smartphone to be used as a virtual wallet for credit and debit cards.

LoopPay's technology, however, purports to sidestep one obstacle that has so far hindered the wide adoption of mobile-payment services: the need for merchants to have to upgrade their checkout devices. Instead, LoopPay says its magnetic induction technology allows a smartphone to be tapped against the magnetic-stripe-reader machine that is already found in the vast majority of U.S. retail stores.

Samsung's service, which is expected to be launched when Samsung unveils its Galaxy S6 smartphone in Barcelona in about a week's time, would allow a consumer to register his credit, debit, gift and loyalty cards onto his Samsung smartphone, and use the handset to make purchases, instead of carrying the cards in a physical wallet.

Analysts say a number of kinks still need to be worked out. But if the technology works as promised, it could give Samsung an edge compared with rivals who have struggled to persuade merchants to upgrade checkout devices to accommodate the near-field communication, or NFC, technology that powers services like Apple Pay and Google Wallet.

Some big U.S. retailers are backing an alternative mobile wallet solution called CurrentC, which will be rolled out this year and relies on a different technology. Some members of that consortium, which include Wal-Mart Stores Inc., Target Corp., CVS Health Corp. and Rite Aid Corp., have quietly turned off their NFC readers to block Apple Pay and other alternatives.

Apple Pay last month said its service is available at more than 220,000 locations in the U.S.--still just a fraction of the country's estimated 12 million checkout points. By contrast, LoopPay chief executive and co-founder Will Graylin estimates that magnetic swipe readers are available at 90% of the country's checkout counters.

"The infrastructure laid out over the last four decades is like the railroad tracks that took decades to lay," Mr. Graylin said. "We're building on the existing rails, and it reaches its destination now."

Bryan Yeager, an analyst at research firm eMarketer, said LoopPay's solution, even if it works as smoothly as promised, could prove to be no more than a "stopgap measure"--particularly if merchants end up embracing NFC card readers that can support Apple Pay and other mobile-payment services.

The LoopPay acquisition is the second high-profile deal Mr. Eun has led for Samsung in the past six months. Last August, Samsung acquired SmartThings, a Washington, D.C.,-based startup that helps connect home appliances to one another.

Mr. Eun said Samsung first approached LoopPay in late 2013, when LoopPay was but a Kickstarter campaign. The South Korean company invested in July alongside Visa Inc. and Synchrony Financial, the U.S.'s largest issuer of credit cards branded by retailers such as Wal-Mart and Amazon.com Inc.

Margaret Keane, president and chief executive of Synchrony Financial, said that while her company, formerly the consumer retail arm of General Electric Co., works with other mobile payment services like Apple Pay, she was impressed by LoopPay's focus on making things easy for retailers.

"They really understood the merchant, and that piece has been missing," she said in an interview.

Christophe Uzureau, a vice president of research at Gartner who specializes in mobile payments, said the key question would be whether Samsung has "the muscle and the commitment" to build a full-scale platform and to foster the right relationships with financial-services firms, and not offer a product that is "purely a reaction to Apple Pay."

Write to Jonathan Cheng at jonathan.cheng@wsj.com

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