Japanese stocks shot lower on Wednesday, as Tokyo was weighed by the yen's strength, on a day of broadly negative trading in Asia.

The Nikkei fell 1.8% in early trading as Japan came back online after a two-day public holiday break to a stronger yen. At Yen101.60, to the dollar, the currency had firmed significantly since the end of last week, when it was trading at Yen102.21.

The yen has strengthened as tensions between Ukraine and Russia have ratcheted up. On the horizon, the markets are looking to Federal Reserve Chairwoman Janet Yellen, who is scheduled to testify on monetary policy later in the day, for comments that could impact the dollar side of the currency pair.

"The stubbornly strong yen, and uncertainty over how much of a bite the [April 1] national consumption tax hike will take out of the economy, continue to plague stocks, and this will not end until definitive figures come out allaying the jitters," said Norihiro Fujito, senior investment strategist at Mitsubishi UFJ Morgan Stanley Securities.

Elsewhere, the region was coming back to life after a quiet session on Tuesday, when much of the region was closed for public holidays. Japan, Hong Kong and South Korea were all closed in the previous session. There was a negative overnight lead from the U.S., where Wall Street ended lower due to falls in small high-growth stocks and Internet stocks.

Australia's S&PASX 200 lost 0.6% and South Korea's Kospi dropped by 0.3%.

Chinese economic data will be in focus for the rest of the week, with trade data coming on Thursday, which will be followed by inflation numbers on Friday. At the beginning of the week, data showed further weakness in China's manufacturing sector, which weighed on Hong Kong stocks Monday.

Bradford Frischkorn contributed to this article.

Write to Daniel Inman at daniel.inman@wsj.com

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