The Justice Department and Macmillan have reached a settlement over the antitrust lawsuit relating to e-book pricing, which means book retailers will be able to discount the digital titles of all major publishers in the U.S.

Macmillan is the last of five major publishers targeted in a Justice Department antitrust lawsuit last year to settle. Three publishers settled in April last year, while a fourth, Pearson PLC's (PSO, PSON.LN) Penguin Group (USA), settled in December. Penguin settled in advance of its planned merger with Bertelsmann SE & Co.'s Random House publishing unit expected to be completed later this year.

The agreement Friday leaves Apple Inc. (AAPL) as the only remaining defendant outstanding in the government's lawsuit. A spokeswoman for Apple declined comment.

The suit alleged the publishers and Apple colluded to raise e-book prices at a time when Amazon.com Inc. was selling new digital best sellers for only $9.99. The government suit noted that e-book best-seller prices subsequently rose to $12.99 and $14.99 following the alleged collusion.

The publishers and Apple denied the allegations.

Terms of Friday's settlement, which must be approved by a court, call for Macmillan to allow e-book retailers to discount its titles within three business days of agreeing to the settlement, even if it hasn't struck new contracts with those retailers. That discounting will be in place for 23 months starting from Dec. 18, 2012, the day on which Penguin settled.

In a statement addressed to authors, illustrators and agents, John Sargent, chief executive of Macmillan, wrote that he settled "because the potential penalties became too high to risk even the possibility of an unfavorable outcome."

Mr. Sargent, who noted in his statement that he continued to believe that Macmillan hadn't done anything wrong, said that the Penguin settlement was a turning point. He also said that the settlement doesn't include an admission of guilt. Macmillan is a unit of Verlagsgruppe Georg von Holtzbrinck GmbH.

Write to Jeffrey A. Trachtenberg at Jeffrey.Trachtenberg@wsj.com

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