By Robin Sidel And Liz Hoffman 

Another blue-chip American company is in the cross hairs of an activist investor.

Hedge fund ValueAct Capital Management LP has taken a roughly $1 billion stake in American Express Co., according to a person familiar with the matter, putting added pressure on the card giant following a series of setbacks in recent months.

Based in San Francisco, ValueAct has built a reputation as a friendly activist and founder Jeffrey Ubben has said he prefers to work behind the scenes with companies rather than clash in public with management. It isn't clear whether ValueAct is pressing for changes at AmEx, which has a market capitalization of about $75 billion and has been led by Chief Executive Officer Kenneth Chenault since 2001.

AmEx shares were down about 19% this year before Friday, in part because the firm announced in February that it would lose a lucrative partnership with warehouse club Costco Wholesale Corp. next year. AmEx shares jumped 6% after Bloomberg News reported the ValueAct stake.

"ValueAct is a well-respected firm," AmEx said in a statement. "We have been speaking with them, as we do with other investors, and look forward to continuing a constructive dialogue."

A person familiar with the situation said that ValueAct hasn't made any demands upon AmEx and that the conversations, which have included Mr. Chenault, began earlier this year.

ValueAct's position is the latest example of activist investors moving up the corporate food chain toward larger targets. So far this year, activists have taken stakes in 17 companies with market capitalizations over $10 billion at the time the position became public, a full-year record and more than double the year-to-date average since 2006, according to FactSet.

Activists have gone after big companies before-- William Ackman's Pershing Square Capital Management LP, for example, agitated for change at Procter & Gamble Co. in 2012--but they have stepped up the pace.

Just this week, Mr. Ackman's Pershing Square disclosed a 7.5% stake in snacks company Mondelez International Inc., which has a market capitalization of $75.3 billion, and Daniel Loeb's Third Point LLC unveiled a 7% stake in pharmaceuticals company Baxter International Inc., with a market value of $22.8 billion. Other big companies contending with activists this year include Macy's Inc., Citrix Systems Inc. and Bank of New York Mellon Corp.

Their ambitions have been fueled by their growing financial firepower. These funds now oversee about $127.5 billion, up from $65.5 billion at the end of 2012, according to HFR. Their increased size helps them put more money behind their ideas and in some ways necessitates bigger swings. ValueAct manages about $20 billion, according to a person familiar with the matter.

ValueAct's roughly $1 billion stake represents about 1.3% of AmEx shares. Positions under 5% aren't required to be quickly reported in regulatory filings.

The move by ValueAct comes at a particularly rough time for AmEx, which said in February that it was unable to reach agreement with Costco on a deal to continue their 16-year relationship.

AmEx has been the only credit card accepted at Costco, and the companies' co-branded credit card accounts for 8% of AmEx's customer spending. Costco cards represent roughly one in every 10 AmEx cards in circulation.

A week later AmEx lost an antitrust lawsuit that will allow merchants to start steering customers to use cheaper cards. The loss was particularly damaging to Mr. Chenault, who had refused to settle the case.

In May, AmEx President Edward Gilligan, who had been viewed as a potential successor to Mr. Chenault, died on a plane after a business trip to Japan.

Mr. Chenault, 64 years old and one of the longest-tenured leaders in the financial services industry, has tried to reassure investors and analysts that the company will be able to bounce back from the recent blows.

Bill Smead, a money manager who started buying AmEx shares in the first quarter, doesn't think ValueAct will demand big changes.

"My guess is that they just have a lot of confidence of where the value of the business is going to be in the next three years," said Mr. Smead, chief executive and chief investment officer of Smead Capital Management, a Seattle-based asset management firm with $1.9 billion in assets under management. The firm held 615,756 shares of AmEx as of Aug. 5, worth around $46 million.

ValueAct typically seeks to maintain a low-key profile, a stance Mr. Ubben has attributed to an uncomfortable turn in the spotlight as chairman of Martha Stewart's home-goods company.

ValueAct's largest position as of March 31 was a 4.4% stake in Valeant Pharmaceuticals International Inc., now worth $3.7 billion. It has held shares of the drug maker since 2006, coinciding with a nearly 900% rise in the company's shares.

The firm typically seeks only one board seat at companies and fills it with Mr. Ubben or President Mason Morfit, unlike other activist funds that seek bigger representation and tap independent nominees. Last year, The Wall Street Journal calculated that of the 32 public companies in which ValueAct joined the board, half changed their chief executives afterward.

ValueAct has been busy lately. Rolls-Royce Holdings PLC said on Monday that it is having "constructive discussions" with ValueAct, following the engine maker's disclosure that the investment firm had taken a 5.4% stake in the company worth about $866 million.

Write to Robin Sidel at robin.sidel@wsj.com and Liz Hoffman at liz.hoffman@wsj.com

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