By Gabriele Steinhauser in Brussels and James Marson in Moscow 

The European Union's competition regulator plans to file formal antitrust charges against Russia's state-owned gas company OAO Gazprom on Wednesday, a person familiar with the matter said Monday, a step set to escalate the standoff between Europe and Moscow.

The European Commission started a formal investigation of Gazprom's business practices in some eastern and southern European countries in September 2012, saying it suspected the company of abusing its dominant position in those countries' natural-gas supply. The bloc's competition commissioner, Margrethe Vestager, said in February that she was ready to file formal charges against Gazprom in a "relatively short time span."

The person familiar with the commission's case against Gazprom said that the charge sheet, known as a statement of objections, against the company has been put on the agenda of the commission meeting Wednesday and that no resistance was expected. A spokesman for Ms. Vestager declined to comment.

Gazprom spokesman Sergei Kupriyanov couldn't be reached for comment.

Russian government and Gazprom officials have denounced the EU's probe as politically motivated and aimed at forcing the company to reduce its prices. Europe is Gazprom's most lucrative market, and the company accounts for around one-third of EU gas imports, making it the largest outside supplier. Some countries in the Baltics and southeastern Europe rely entirely on shipments from the Russian company.

With the planned charges, Ms. Vestager is escalating another antitrust case against a major company in a big country just one week after the commission filed formal charges against U.S.-based Google Inc. The case against Gazprom could potentially result in multibillion euro fines against the company.

The commission has tread carefully on the Gazprom case over the past year, amid concerns that moving ahead with charges could make it more difficult to reach a diplomatic solution with Moscow over its actions in eastern Ukraine.

But when Ms. Vestager took office in November she said she would take a fresh look at the investigation and not be steered by political considerations. At the same time, progress on the Minsk Agreement, the EU's peace plan for Ukraine, has been slow, indicating that a resolution of the broader conflict is likely still some time off.

When the commission opened its formal investigation into Gazprom's practices 2 1/2 years ago, it said it was suspecting the company of unfair behavior in three areas. Firstly, the commission has been examining whether Gazprom is preventing some countries from re-exporting gas they have bought from the company.

The commission has also been investigating whether Gazprom has tied some aspects of its contract with customers--such as the price it charges for gas--to cooperating in other business areas, including the building of new pipelines.

And finally, the commission has been looking into Gazprom's practice of tying the price it charges for gas to international oil prices.

After the formal investigation was announced, Russian President Vladimir Putin hit back with a decree preventing the company from disclosing information to foreign regulators without government permission. But EU investigators had already gathered materials in a string of surprise raids on Gazprom subsidiaries and large European national energy suppliers a year earlier.

Before talks broke down last year, shortly after Russia's annexation of the Crimean peninsula from Ukraine, Gazprom and EU competition regulators had come close to settling the first two elements of the case, according to people familiar with the investigation. A lot less progress had been made on the oil-price link--partly because other gas companies have pursued a similar pricing policy--and experts have questions whether the commission would be able to build a case against Gazprom on that front.

The person familiar with the commission's case didn't say on what parts of the investigation charges would be filed Wednesday. Charges could be limited to just parts of the initial probe and narrowed down from the eight countries the commission was originally examining. That could reduce the potential fines Gazprom would face.

By sending a formal charge sheet to Gazprom, the commission doesn't rule out the possibility of settling--a strategy that the company had publicly embraced before talks stopped and that would allow it to avoid costly fines. A person familiar with Gazprom said Monday that the company had signaled to the commission its willingness to settle the case as recently as last week, but that no formal talks had taken place since last year.

"There is a very strong argument [for Gazprom] to settle," said Alan Riley, a professor at City Law School in London who specializes in energy issues, adding that a legal decision against the company and fines would be "pretty catastrophic from a business point of view."

But Mr. Riley cautioned that political considerations could prevent Gazprom, which is controlled by the Russian government, from agreeing to an amicable solution. "Whatever way it is put, it would be seen as some kind of climb-down" by Moscow, he said.

The commission has been working to reduce the EU's dependence on Gazprom and Russia. Late last year, Moscow said that an EU law that forces pipeline owners to open up their infrastructure to rival suppliers had forced it to drop a major new transport route that would have bypassed Ukraine.

Instead, Russia is now trying to build an alternative pipeline via Turkey, known as Turkish Stream, and Moscow and Gazprom have been grooming some EU governments for support. Gazprom's chief executive, Alexei Miller, is scheduled to be in Athens on Tuesday to meet Prime Minister Alexis Tsipras, who is currently struggling with the rest of Europe over the terms of continued financial aid.

Mr. Tsipras's leftist government has asked Russia for a discount on its gas and discussed the possibility of continuing Turkish Stream into Greece, which would give it a crucial link into EU territory.

European customers have in recent years squeezed discounts from Gazprom amid competition from liquefied-natural gas deliveries and cheap American coal shipments to power producers. The recent drop in oil prices has also been a boon for those whose contracts are still oil-linked.

Gazprom has said that regulatory pressure in Europe is accelerating its attempts to sell its gas in Asia. Last year, Russia signed a long-delayed gas contract with China, although analysts from Sberbank CIB say the projected pipeline looks expensive, and that the company would be better off seeking another route for deliveries.

Tom Fairless in Brussels contributed to this article.

Write to Gabriele Steinhauser at gabriele.steinhauser@wsj.com and James Marson at james.marson@wsj.com

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