By David Benoit And Lauren Pollock 

DuPont Co. named two new directors in a board revamp that excludes nominees of Nelson Peltz's Trian Fund Management Corp., ratcheting up the tension in a battle with the activist investor.

The chemical giant said Edward Breen, chairman of Tyco International PLC, and James Gallogly, a former chief executive of LyondellBasell Industries NV, would join its board effective immediately. DuPont also revealed it had rejected Mr. Peltz and two of Trian's three other board nominees in its proxy fight with the company.

In an attempt to settle the fight over the board of the Delaware chemicals conglomerate, DuPont said it offered on Wednesday to consider one of Trian's director nominees along with Messrs. Breen and Gallogly, according to a letter to Mr. Peltz the company released Thursday.

"Unfortunately, you insisted--while refusing to hear the details of our proposal--that you will not consider any proposal that does not include you personally being added to the DuPont Board," DuPont's letter said.

DuPont judged John Myers, the former CEO of GE Asset Management, part of General Electric Co., to be Trian's one qualified nominee, according to people familiar with the matter.

Trian called news of DuPont's new directors "another positive development since Trian's involvement with DuPont began in March 2013"; however, the fund said "there is much more value to be unlocked," noting up to $4 billion in excess corporate costs, among other concerns. For those reasons, the fund said it would continue to push its nominees to join DuPont's board.

DuPont's conglomerate structure and diverse business portfolio have come under scathing attack from Trian over the past 18 months, and the activist said last month that it would seek four seats on DuPont's board. Trian has questioned management and the board's supervision of the company, even amid strong performance for its stock, which has more than doubled in the past five years and outperformed the S&P 500 index.

DuPont shares rose 3.1% Thursday to $76.

DuPont said it reviewed all of Trian's proposed nominees and met with Mr. Peltz on Wednesday in Chicago to try to reach a settlement, according to the letter, signed by Chief Executive Ellen Kullman and Alexander Cutler, the lead independent director. The letter also again rejected breaking up the company, a step Trian has advocated.

Trian's other two nominees are Arthur Winkleblack, former chief financial officer of H.J. Heinz Co., and Robert Zatta, acting CEO and CFO of chemical company Rockwood Holdings Inc.

Trian has argued the company's share value could effectively double if it split itself into three--with one business in agriculture and nutrition, another in industrial materials and a third for performance chemicals, which produces materials that go into things such as nonstick frying pans and house paint. DuPont is in the process of spinning off the performance-chemicals business.

DuPont has fired back that its board and Ms. Kullman are already reshaping the company and performing well. Several companies have added independent board members in the midst of activist fights of late, which can serve as a way to appease the investors and defuse the battles.

Some DuPont shareholders and other observers have previously given the company high marks, but also left open the possibility they would side with a move to get fresh blood on the board. Shareholders will now vote on whether the revamped board is enough or whether they will still want Mr. Peltz and his slate.

Trian, in its release Thursday, said, "While DuPont frames Trian's insistence that Nelson Peltz be added to the board as a negative, we believe fellow stockholders want our nominees on the board, specifically Mr. Peltz."

Mr. Breen has experience with corporate breakups, which could bolster an argument by DuPont that it is sufficiently considering the possibility of one and doesn't need the added supervision of Mr. Peltz and others. Mr. Breen was CEO of Tyco International from 2002 to 2012, during which time he oversaw two breakups of the company, resulting in the spinoffs of Covidien PLC, TE Connectivity Ltd. and ADT Corp. and the merger of Tyco Flow Control with Pentair, DuPont said.

For his part, Mr. Gallogly led LyondellBasell through bankruptcy and a restructuring at the chemical company that cut its staff by 20%. LyondellBasell exited bankruptcy in less than a year and is now the third-largest independent chemical manufacturer in the U.S., DuPont said.

The appointments come as two current directors, Curtis Crawford and Richard Brown, leave the DuPont board to eventually serve as directors at Chemours Co., the performance-chemicals business that is being spun off.

Jacob Bunge and George Stahl contributed to this article.

Write to David Benoit at david.benoit@wsj.com and Lauren Pollock at lauren.pollock@wsj.com

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