By Jeffrey T. Lewis and Rogerio Jelmayer 

SAO PAULO--Brazil's antitrust agency is investigating banking giants Citigroup Inc., HSBC Holdings PLC, Deutsche Bank AG and a long list of their peers on suspicion of forming a cartel to manipulate the exchange rate of the Brazilian currency, the real.

There are "strong indications" of the use of anticompetitive practices in the foreign-exchange market by the three big banks and 12 other U.S. and overseas lenders, the agency, known as CADE, said Thursday. The agency also named 30 individuals in the investigation.

CADE said there was evidence the banks and the individuals worked together to fix the exchange rate, coordinate the buying and selling of currencies, manipulate the Brazilian central bank's PTAX reference exchange rate and impede the operations of other banks operating in Brazil's foreign-exchange market, among other things.

HSBC in Brazil, Citigroup and Deutsche Bank declined to comment on the allegations. Among the other institutions named by CADE, J.P. Morgan Chase & Co., Morgan Stanley and Credit Suisse Group AG had no immediate comment, and Barclay's PLC, Royal Bank of Scotland Group PLC and UBS AG declined to comment.

About $240 billion was traded on Brazil's spot market in May and about $311 billion was traded on its futures market in the same month. That includes purchases of dollars by Brazilians heading to Florida for vacation and U.S. companies buying reais to invest in Brazil.

The size and scope of the investigation are unprecedented, according to Reginaldo Galhardo, foreign-exchange manager at the Treviso currency brokerage in Sao Paulo.

"This is the first time I've seen an operation involving so many banks," he said. "It was almost certainly motivated by investigations in other countries."

Earlier this year, five of the banks under investigation in Brazil pleaded guilty to similar offenses in the U.S., and agreed to pay more than $5 billion in combined penalties. Four of the banks--Barclays, Citigroup, J.P. Morgan and Royal Bank of Scotland--pleaded guilty to conspiring to manipulate prices in the $500-billion-a-day market for U.S. dollars and euros.

The fifth bank, UBS, received immunity in the antitrust case but pleaded guilty to manipulating the London interbank offered rate, or Libor.

CADE didn't say if its investigation is related in any way to the U.S. investigation.

The Brazilian antitrust agency has close relations with authorities in other countries and is probably getting help from them because the banks involved are all international companies, according to Gesner Oliveira, a former head of CADE. If charged, tried and found guilty of the offenses CADE says they have committed, the banks could be fined as much as 20% of their annual revenue, an amount that could be doubled if any of the banks were found to be repeat offenders, he said.

"Now CADE will carry out a detailed investigation of the case and any resolution won't be fast," Mr. Oliveira said. "At best it could take several months, and at worst it could last years."

The alleged crimes in Brazil took place from at least 2007 to 2013, CADE said. The banks coordinated their actions using chat services provided by Bloomberg LP, and some of the participants referred to the group as "the cartel" or "the mafia," according to CADE. At least one of the participants in the alleged cartel is cooperating with authorities, CADE said.

Bloomberg declined to comment. Bloomberg is a competitor of The Wall Street Journal in the provision of financial news.

The banks under investigation collaborated to influence certain reference indexes that affect the exchange market such as the Central Bank of Brazil's PTAX rate, CADE said. The PTAX is used by big companies, banks and investors to set exchange rates in contracts and for other transactions.

The banks also shared information on clients' orders, futures contract prices and the size of operations already carried out, among other things, according to CADE.

Aruna Viswanatha in Washington contributed to this article.

Write to Jeffrey T. Lewis at jeffrey.lewis@wsj.com and Rogerio Jelmayer at rogerio.jelmayer@wsj.com

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