By Douglas MacMillan 

Marissa Mayer is finally seeing some growth at Yahoo Inc., but it is coming at a cost.

The company on Tuesday reported its revenue--minus commissions paid to partners for Web traffic--edged higher in the second quarter, only the second time sales have grown in the past five periods. Revenue from display ads, excluding the traffic costs, rose 3.3%, to $406.7 million.

But the company posted a loss for its most recent quarter, as the cost of acquiring traffic jumped sharply to $200.2 million from $43.8 million in the year-earlier period.

Those costs are adding up as Ms. Mayer, three years into her tenure as chief executive of the aging Internet portal, bets heavily on emerging areas including mobile and video ads. The growing portion of revenue Yahoo is paying its partners "isn't a healthy sign," said Mark Mahaney, analyst at RBC Capital Markets.

Revenue growth from "Mavens"--a financial metric the company introduced earlier this year to track mobile, video, native and social ads--is growing. Mavens revenue makes up about 32% of the company's total, growing 60% to $399 million in the second quarter. That was up from 58% growth in the previous quarter.

Yahoo shares fell 1.3% in after-hours trading, to $39.20.

Total revenue rose 15%. Yahoo reported its highest quarterly revenue increase in almost nine years and display advertising saw the "most substantial" revenue increase since 2010, Ms. Mayer said.

Ms. Mayer has made some of her boldest bets on new mobile and video offerings this year. In May, Yahoo announced it had won the exclusive rights to the National Football League's first streaming-only broadcast of a football game, a deal costing the company more than $20 million, a person familiar with the matter said at the time.

Earlier this month, Yahoo unveiled a new foray into legal online gambling with a retooled version of its fantasy-sports mobile app that lets users wager real money daily and weekly against their friends and in bigger online tournaments.

Yet search remains a priority for Ms. Mayer, who in June unveiled a new version of Yahoo's search engine for mobile phones. The company earlier this year renegotiated its partnership with Microsoft Corp., a deal that gave Yahoo more control over the search results and ads it shows on desktops and mobile phones.

Search revenue, after commissions paid to partners, fell 3% in the second quarter, to $415 million.

Yahoo continues to lose ground to Internet ad rivals Google Inc. and Facebook Inc. This year, Yahoo will claim 4.6% of the $27 billion market for online ads in the U.S., down from a 5.5% share last year, estimates eMarketer Inc. Facebook will grow its share to 25.2%, up from 23.8% last year. Google will drop slightly to 13% this year, down from 13.7% in 2014.

Yahoo posted a loss of $21.6 million, or 2 cents a share, from a year-earlier profit of $269.7 million, or 26 cents a share. On an adjusted basis, earnings were 16 cents, falling just below analyst estimates of 18 cents a share.

Investors continue to look for signs that Yahoo's planned tax-free spinoff of its shares in Alibaba Group Holding Ltd. will happen later this year as planned. Ms. Mayer and finance chief Ken Goldman will likely face questions about the status of that deal on a call with analysts Tuesday.

Write to Douglas MacMillan at douglas.macmillan@wsj.com

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