By Deborah Gage And Jeff Elder 

In a packed San Francisco courtroom earlier this month, John Doerr looked exhausted, at times gloomy, after hours of testimony in which he defended the Silicon Valley venture-capital firm where he has worked for 35 years against allegations of sex discrimination and retaliation.

The billionaire investor explained why Kleiner Perkins Caufield & Byers had placed a male partner on the board of startup RPX Corp. instead of his accuser, Ellen Pao, who pushed for the investment.

Randy Komisar, the male partner, "needed a win; Kleiner needed a win; everybody needs wins," said Mr. Doerr, facing Ms. Pao, who sat some 15 feet away in front of her mother. "I could use some wins."

Wins in this sense refer to the kind of smash-hit investments Kleiner became famous for making, such as early bets on Google Inc. and Amazon.com Inc.

But a courtroom win over Ms. Pao might do little to repair Kleiner's reputation.

A six-man, six-woman jury is set to begin deliberations Wednesday in San Francisco Superior Court after more than four weeks of testimony in the case. The trial exposed business secrets, details of infighting among Kleiner's partners, amorous liaisons in parking lots and allegations of dirty talk about porn stars on a private jet.

In interviews, some entrepreneurs and limited partners--the pension funds, endowments and family offices that are the lifeblood of any venture fund--have expressed concern about Kleiner.

Ms. Pao sued Kleiner in 2012, alleging that she was passed over for promotions, and then fired, after complaining about being discriminated against because she was a woman. Kleiner has denied her claims, and sought more help from Brunswick Group, the crisis-management firm it hired as the lawsuit sparked a debate about Silicon Valley sexism.

The trial put Kleiner under intense media scrutiny. The 150-seat courtroom was crowded with the technology press and more than a dozen reporters from major news outlets, including several working for Chinese-language media, who parsed the testimony via live blogs and on Twitter.

Photographers lurked outside the women's bathroom for a glimpse of the 45-year-old Ms. Pao.

Most days, a Kleiner marketing partner and three spokespeople sat in the courtroom to monitor stories and complain to reporters when they thought coverage was biased or inaccurate. Kleiner attorney Lynne Hermle, whose cross-examination of Ms. Pao attracted a standing-room audience, also confronted reporters about their stories.

Mr. Doerr, 63, and more than a half-dozen current partners at Kleiner were called to testify, including Mr. Komisar, who was pressed on the stand to justify his decision to give Ms. Pao a book of poetry for Valentine's Day that contained erotic illustrations. Mr. Komisar said it was an innocent gift from his wife and related to the fact that he and Ms. Pao shared an interest in Buddhism.

Despite Kleiner's attempts to manage the story--and emphasize that its investment staff includes more women than the industry average--the case has ignited controversy among those associated with Kleiner.

"It's sad to see it; there are no two ways about it," said Bob Ackerman, whose venture firm Allegis Capital invested alongside Kleiner in at least three startups last year.

"When you see something like this, you can't help but do a little self-assessment..." he said. "I think a lot of things look worse in retrospect than they did at the time."

An investor who has backed Kleiner funds said the limited-partner community is "disappointed and saddened" by the case and suggested that Kleiner use the opportunity to restructure.

Kleiner declined to comment on the case while it is continues.

Entrepreneurs who have raised money from Kleiner or considered doing so are divided in their support of the firm. Some founders said they would take money from Kleiner no matter what, while others stated they would hesitate to do so pending the verdict.

The chief executive of one fast-growing company in which Kleiner is a major investor said that while the firm is an "excellent investor," he is worried about his affiliation with it. "I hope it's as they say and there's no wrongdoing," he said.

Other founders were kinder in their assessment of the firm. "I've spent plenty of time with plenty of VCs, and there are gender issues in the tech world, but they're not happening at Kleiner," said Tracy DiNunzio, CEO of Tradesy Inc., an online clothing marketplace that raised money from Kleiner last May and granted Mr. Doerr a board seat.

As Kleiner copes with the turmoil that comes with a public hearing, it also continues to battle a perception that it has lost its way as an investor.

A decade ago, Mr. Doerr seemed like he could do no wrong after his prescient bets on Google, Amazon and Netscape Communications turned Kleiner into a powerhouse with strong political connections.

But by 2006, a year after the firm distributed at least $2 billion in Google stock to its limited partners, Mr. Doerr decided it would charge hard into clean technology, dedicating hundreds of millions of dollars to the sector over the next several years.

That proved to be a costly mistake, with Kleiner suffering massive losses in companies such as solar-panel producer MiaSole and the electric-car maker Fisker Automotive.

Meanwhile, Kleiner missed the rise of social media and mobile technology and had to play catch-up with later-stage investments in companies such as Facebook Inc. and Twitter Inc.

Since 2013, Kleiner has acknowledged its missteps to limited partners, cut the management fees to run its fund, scaled back the size of its main fund to $450 million last year from a high of $700 million in 2008, and reduced its senior investor staff by 33% to eight, according to people familiar with the matter.

"We needed to get smaller to make better decisions," Mr. Doerr testified.

Last year, the firm scored a big win when Google paid $3.2 billion to acquire household device maker Nest Labs, in which Kleiner was an early investor. Venture-capital insiders say Kleiner is still ranked in the upper tier of venture firms but no longer is considered the must-have backer by young startups.

Kleiner is an investor in 17 private companies valued at $1 billion or more, according to Dow Jones VentureSource, though only three of those investments were made at an early stage when a company's success is less assured but investment returns are potentially greater.

By comparison, its rival Sequoia Capital is an early investor in eight of its 17 billion-dollar companies.

In an effort to close a perceived generation gap, Kleiner tried to merge with a younger firm, Social+Capital Partnership, but those talks ceased, according to a person familiar with the talks.

Kleiner continues to have "lots of conversations with lots of people," this person said.

Like most venture firms, Kleiner doesn't have a conventional human-resources department, only a policy covering discrimination and retaliation. Stephen Hirschfeld--who was hired by Kleiner to investigate Ms. Pao's complaints about a former partner with whom she had a consensual affair--testified that partners also didn't have a clear idea of how to succeed at the firm.

Crisis-communications expert Sam Singer said Kleiner should change its culture, adopt new human-resources policies and procedures and promote women and minorities to repair its standing.

"The legal trial isn't about [Kleiner]," Mr. Singer said. "It's about how the public will ultimately judge venture-capital firms above and beyond financial success."

Write to Deborah Gage at Deborah.Gage@dowjones.com and Jeff Elder at jeff.elder@wsj.com

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